???? ??? ??? ?? ? ???? ????
專利匯可以提供???? ??? ??? ?? ? ???? ????專利檢索，專利查詢，專利分析的服務(wù)。并且PURPOSE: A method for eliminating heavy metal from soil using an amino acid-based liquid fertilizer is provided to improve the eliminating efficiency of the heavy metal by increasing effective microorganism in the soil. CONSTITUTION: An amino acid-based liquid fertilizer accelerates the biological elimination of heavy metal from soil. 0.05 to 5ml/m^2 of the amino acid-based liquid fertilizer is sprayed to the soil. The amino acid-based liquid fertilizer is diluted to 200 to 1000 times. The amino acid-based liquid fertilizer is sprayed for two or three times per week. A heavy metal eliminating agent with respect to the soil is based on the amino acid-based liquid fertilizer as an effective component.，下面是???? ??? ??? ?? ? ???? ????專利的具體信息內(nèi)容。
???? ??? ??? ?? ? ???? ???? {A Method for Removing Toxic Metals in Soil Using Amino Acid Liquid Fertilizer}
? ??? ???? ??? ??? ??? ?? ???, ?? ???? ??? ???? ???? ????? ??? ???? ?? ? ???? ????? ?? ???.
?? ?? ? ???? ???? ?? ???? ??? ???? ???, ???? ?? ?? ??? ???? ??. ???? ???? ??? ?? ?????? ????? ???? ?????? ???. ?????? ??????? ?????, ??? ??, ????? ??, ? ?? ?? ???, ??? ???? ??? ??? ??? ???, ??? ?? ?? 2? ????? ??? ? ???, ??? ??? ??? ??(Kumino ?, 2001). ??? ??? ????? ?? ?? ???? ?? ???? ???? ? ???? ???? ????? ?? ? ???? ????? ????? ???? ?????? ????? ?? ???? ??, ?????, ?? ??? ??? ????? ??? ??? ????? ???? ?? ????(Kumino ?, 2001).
1. ???? ????? ?? ??? ?? ??
???? ????? ????? ???? ????? ???? ????, ????? ?? ???? ???? ????? ???? ?? ??? ??????? ??? ?? ????? ??? ??? ????? ?? ??? ?????? ????(??? ?, 2001). ???? ????? ?? ??? ?? ???? ????? ????? ?? ??? ?? ??(Wade et al ., 1993; White et al ., 1997; Bolton Jr. and Gorgy, 1995), ???? ????? ?? ??? ?? ??(Chen and Hao, 1998), ??? ? ??????? ?? ??? ?? ??(Pongratz and Heumann, 1999; Bolton Jr. and Gorgy, 1995), ?? ???? ????? ?? ?? ??(Diels et al . 1999), ???? ?????? ?? ??? ?? ??(White et al ., 1998; Bolton Jr. and Gorby, 1995) ?? ??.
2. ??? ??? ????? ?? ??? ?? ??
??? ??? ?? ??? ?? ??? ??? ?????? ??????? Phanerochaete chryosporium ? ??? ??? Cd, Pb, ??? Cu ?? ??? ????? ?? ?? ??(Day et al. , 2001)? Yu ?(2000)? Durvillaea potatorum ? ???? ?? ?? ?? ????(macroalgae)? ??? ?????(biosorbent)? ???? ??? ?? ??? ??????, ?? Cladophora crispate ??(Ozer et al ., 2000), Durvillaea , Laminaria , Ecklonia ??? Homosirar ??(Figueira et al ., 2000), Chlorella sorokiniana ? ??? 11?? ??(Chong et al ., 2000), Microcystis aeruginosa (Pradhan et al ., 1998) ?? ??? ?? ?? ????.
3. ???? ??? ?? ??
?? ????? ?? ????? ?? ?? ????? ?? ???? ??? ???? ??. ??? ???? ??? ??? ??? ??? ??? ??? ?? ??? ?? ???, ?? ???? ????? ???? ?????. ?? ?? ??? ???? ??? ???? ???, ??? ??? ??? ?? ??? ?? ?? ?? ????. ?? ?? ?? ? ?? ????(Muller, 1991; Rodgers, 1993; Causin, 1993)? ??? ???? ?? ????? ?? ??? ????? ??? ?? ??, ?? ?? ??? ??? ???? ? ????? ?? ??? ?? ??? ?? ??? ?? ? ?????? ??? ??? ?? ??? ???? ??? ????. ?? ????? ?? ??? ?? ????, ???? ???? ???? ?? ???? ? ? ??? ??? ?? ???(Perssom, 2002; Perssom, 2001).
???(2003)? ??? ???? ??? ????? ?, ??? ??? ??? ??? ??? ?? ??????, ??? ???? ??? ?? ???? ?? ?????? ?? ? ?????? ???? ?? ?????, ??? ???? ??? ?? ???? ?? ????? ?? ??, ?? ?? ? ??? ??? ???? ?? ???, ??? ???? ???? ?????? ?????. ?? ???? ??? ??? ?? ?? ?? ? ?? ???? ???? ??? ??? ??? ?? ??? ? ? ???? ???.
?? ???(2006) ? ?? 10-0743525???? ??? ?? ? ??? ?? ? ?? ?? ??? ?? ???? ??? ??? ??? ?? ??? ??, ???? ?? ????? ???? ???? ???? ????? ?? ???? ?? ? ?? ??? ????? ????? ??? ?? ??.
[????]
???, ???. 2006. ??? ?? ??? ??? ??? ?? ??? ????? ????. ??????????. 34(2):83-93.
???. 2006. ???? ????? ?? ??? ?? ???? ??? ??? ?? ??. ????? ????.
???, ???, ???. 1989. Metallothionein ???? ??? ??? Saccharomyces cerevisiae? ??? ??? ??. ????????. 11(5):543-549.
???, ???, ???, ???, ???, ???, ???, ???. 2004. ?? ????, 5th., ???????, ??.
???, ???, ???. 2003. ???? ??? ???????(Bentgrass)? ??? ??? ??? ??. ???????. 17(4), 147-154.
???, ???. 1991. ?????? ?? ????? ???? ??. ????????. 10(2), 158-166
???, ???, ???. 2001. ?? ??? ???? ??? ?? ?????? ?? ?? ? ??. ??????. 19(2):93-99.
???, ???, ???. 2003. ??? ?? ??? ???? ??? ??. ???????. 36(3), 127-134.
???, ???. 2000. ??? ???(???)? ??? ??? ??. ?????? ?????. 27(2):99-117.
???. 2000. ???? 2000.
Amann, RI, W. Ludwig, and KH Schleifer. 1995. Phylogenetic identificaiton and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59, 143-169.
Bitton, G. 1994. Biotransformation of Xenobiotics and metals In:Wastewater Microbiology, John Wiley & Sons, 311-331.
Bolton JH and YA Gorby. 1995. An overview of the bioremediation and bioreduction of inorganic contaminants. pp.1-16. In Bioremediation of Inorganics(Hinchee, RE, JL Means &DR Burris eds.). Battlle press, Columbus.
Causin, HF and AJ Barneix, 1993. Regulation of NH4+ uptake in wheat plant : Effect of root ammonium concentration and amino acids. Plant Soil. 151, 211-218.
Chong AMY, YS Wong and NFY Tam. 2000. Performance of different microalgal species in removing nickel and zinc from industrial wastewater. Chemosphere 41:251-257.
Cladopphora crispata in a two-staged reactor. J Chem. Technil. Boitechnol. 75:410-416.
Day R, A Denizli and My Arica. 2001. Biosorption of cadmium(II), lead(II) and copper(II) with the filamentous fungus Phanerochaete chrysosporium. Bioresource Technol. 76:67-70.
Diels L, M De Smet, L Hooyberghs and P Corbisiter. 1999. Heavy metals bioremediation of soil. Mol. Biotechnol. 12:149-158.
El Amin NM, HS Hanson, B. Petterson, B. Petrini, and LV Von Stedingk. 2000. Identification of non-tuberculous mycobacteria: 16S rRNA gene sequence analysis vs. conventional methods. Scand J. Infect Dis. 32, 47-50.
Gadd. G, M. 1992. Biosorption, J. of Chem. Technil. Biotechnol., Vol. 55, No. 174, Iss. 3. 302-304.
Kumino, T., K. Seaki, K. Nagaoka, H Oyaizu, and S. Matsumoto. 2001. Characterization of coper-resistant bacterial community in rhizosphere of highly copper-contaminated soil. Eur. J. Soil Biol. 37:95-102.
Lingens, F. 1985. Phenylobacterium immobilegen. nov., sp. a gram-negative bacterium that degrades the herbicide chloridazon. Int. J. Syst. Bacteriol 35, 26-39.
Muller, B and B. Touraine, 1991. Inhibition of NO3- uptake by various phloem- translocated amino acids in soybean seedlings. J. Exp. Bot. 43, 617-623.
Ozer A, A Ozer and GDursun. 2000. Investigation of zinc(II) adsorption on Figueira MM, B Volesky, VST Ciminelli and FA Roddick. 2000. Biosorption of metals in brown seaweed biomass. Water Res. 34:196-204.
Perssom. J. and T. N?sholm. 2002. Regulation of amino acid uptake in conifers by exogenous and endogenous nitrogen. Planta. 215, 639-644.
Perssom. J. and T. N?sholm. 2001. Amino acid uptake : a widespread ability among boreal forest plants. Ecology Letter. 4, 434-438.
Pradhan S, S Sin?, LC RAi and DL Parker. 1998. Evaluation of metal biosorption efficiency of laboratory-grown Microcystis under various environmental conditions. J. Microbiol. Biotechnol. 8:53-60.
Rodgers, CO and HJ Bormeix. 1993. The effect of amino acid and amides on the regulation of nitrate uptake by wheat seedlings. J. Pl. Nutr. 16(2), 337-348.
Wade MJ, BK Davis, JS Carlisle, AK Klein and LM Valoppi. 1993. Environmental Transformation of toxic metals. Occup. Med. 8:574-601.
Yu Q, P Kaewsarn, JT Matheickal and W Ma. 2000. Pretreated biomass of marine macroalgae as low cost high efficiency adsorbent for heavy metal ions. J. Chin. Inst. Chem. Eng. 31:411-415.
? ??? ???? ??? ??? ??? ?? ???, ?? ?? ? ???? ??? ??? ???? ??? ??? ??? ???? ?? ???? ??. ? ????? ?? ? ???? ??? ??? ???? ??? ??? ??? ????. ?? ? ????? ?? ? ???? ??? ??? ?? ???? ???? ??? ??? ??? ????.
?? ? ??? ?? ?? ? ???? ??? ??? ???, ? ??? ??? ?? ? ? ?? ? ???.
? ?????,
???? ??? ?? ? ???? ????? ??? ???? ?? ???? ?? ?? ? ???? ????? ????. ?????? ?? ???? ??? ?????? 0.05～5?/㎡? ??? ?? ????.
??, ?? ???? ??? ?????? 200~1,000?? ???? 1? ?? ????, ?? ?????? 1?? ???? 2～3? ????.
??, ? ????? ???? ??? ?????? ?? ?? ? ??? ???? ????.
? ??? ??? ???? ??? ???? ?? ? ?????? ?? ??????? ???? ??? ???? ???? ???? ???? ????? ?? ???? ? ??.
?? ?? ? ???? ???? ?? ??? ???? ??? ???? ???, ???? ??? ??? ???? ?? ???? ?? ????. ? ??? ?? ?????? ???? ??? ?? ??? ???? ??? ??? ???? ?? ??? ?? ???? ??. ? ??? ???? ??? ??? ?? ? ??? ?? ??? ??? ???? ?? ??? ??? ? ?? ????? ?? ? ???? ???? ??, ?? ???? ?? ??? ?? ??? ????.
?? ???? ???? ?? ? ??? ?? ??? ????. ??? ?? ???? ??? ? ??? ?? ????? ???? ?? ???, ? ??? ??? ?? ???? ?? ???? ?? ???.
?? ? ??
1. ????
? ??? ????? ??? ?? ?? ?????? ?? ?? ? ??? ? ?? ?? ??? ???? ??????(ø30㎝×33㎝)?? ?????, ?? ??? 1-1 ????? 1-1 ????? ?????. ?? ??? ????? ??? (?)???? "???????" ???? ??(LFcAA)? ?????. ????????? ??????? ???? ??? A?? ???? ??? ????? ??????? ? ????? ?? 16.39g/?? 41.71g/?? ???? ???. ??? ??? ???? 3CdSO 4 · 8H 2 O, PbCl 2 , ZnCl 2 , ??? CuCl 2 ???? ???? ?????. ???? ???? ?? ???(500??)? ??? ???? ?????, ????? ???? ?? 10?? 3??, ? 60?? 1-1 ??? ???? ??? ?????.
??? 2009? 6? 11?? ???, ?, ??, ??? ?? ???? ????? ??? ??? ??? ??? ? 7??? ???? ?? ?? ????? ???? ??? ??????, ??? ?? ??? ? ? ?? 50? ?? ?? ? ??? ??? ??? ?????.
2. ??? ??
??? ??? ???? ? ?? 900kg?? ??? ? 15kg? ???? ??????, ??? ??? ???? ??? ???? ??? ????? ???? ??? ??? ??? ?? ???? ????(? 1). ??? ???? ???? ???? ???? ???? ??? ??? ????, ?????? ???? ?? ? 7?? ?? ? ??? ?????.
3. ???? ?? ??
A?? ???? ??? ?? 500???? ???? ??????? ?? ?????. 50?? ??? ???? ???? ??? ?? ?????, ?? ??? ??? ?? ???? ???? ??? ???? ?? 6? 18? ?? 10?? ?? ?? ?????. ???? ?? ???? ??? ????? ???? ????, ???? 1㎝ ? ????? 1,000?(?? 2?)? ?????. ?? ??, ????? 5㎝? ? ????? 5,000?(?? 10?), ????? 10㎝? ? ????? 10,000?(?? 20?), ????? 20㎝? ? ????? 20,000?(?? 40?)? ?????.
4. ???? ? ????
1) ?? ? ??? ?? ??
??? ?? ?? ??? ??? ??? ???? ????? ???? ???? 2009? 8? 1?(?? ? 50?) 1?? ?? ?????. ?? ??? ????? ???? ???? ? ??? ???? 5?? ?? ???? ???? ???? ??? ???? ???, ??? ???? ??? ?? ?????. ??? ?? ??? ???? 5~10? ?? ?? ?? ? 2.0㎜?? ?? ??? ??? ??????, ?? ? ??? ??? ICP? ???? ?????.
2) ??? ???(16S rDNA) ??
PCR ??? gel ??? ? ? TOPO TA cloning kit PCR2.1-TOPO vector(Invitrogen, Co., California, USA)? ???? ???? ????? ?? ??? ???. ??? ?????? 16S rDNA? ?? ??? ??? ? 110?? ???? ?????. PCR2.1 vector ?????? rDNA insert?? ?? ????? M13F(GTAAAACGACGGCCAG)? M13R(CAGGAAACAGCTATGAC)? ???? PCR ?????.
??? ???? ABI 3700XL DNA sequencer (Applied Biosystems, Foster, USA)? ?? ????? ???? (?)???(Daejeon, Korea)? ?? ??? ?????.
??? 16S rDNA ?? ???? NCBI(National Center for Biotechnology Information; http://www.ncbi.nih.gov/)? BLAST??? ?? ??? ?? ??? ??? ???? ?? ??? ?????. ???? ??? ??? Clustal X(ver. 1.83) (Thompson, 1997)? ?????.
?? ? ??
1. ?? ? ??? ?? ??
?? ? ??? ??? ?? ???? ??? ??? ??? ??? ??? ??? ??? ??(? 2).
? 2?? ????? ????? ????? ???? ?? ?? ? ????? ??? 40.05㎎/mk, ? 829.50㎎/mk, ?? 176.43㎎/mk, ?? 597.04㎎/mk? ???? ???????? ?·? ??? ??? ??? ????? ?? ?? ??? ??? ??. ???? ?? ?? 50? ? ?? ? ??? ??? ??? ????, ???? ?? ???? ??? ????? ?? ?? ?? 22.6~75.7%? ???? ???. ?? ???? ?? ?? ? ???? ???? ?? ?? ? ??? ??? ??? ????.
2. ?? ? ??? ( 16S rDNA) ??
??? ?? 0～10㎝? ??? ???? ???(C), ???? ?? ???(T) ??? ??? ??? ?? 110?? ???? 16S rDNA clone partial sequence? ?????. 110?? ??? ?? ?????? chromas213 ?????? ??? ? Genebank database? ???? 16S rRNA ??? ?????. 16S rDNA ??? ???? ?? ?? Alpha-, Beta-, Gammaproteobacteria ??? ??? ???? ?? ?????(? 3). BLAST?? ?? ???? ???? 90% ??? ???? ?? ???????, ??? 95% ??? ?? ???? ?? ??? ????.
?? ? ??? ?? ??(? 3) Alpha-, Beta-, Gammaproteobacteria ??? ??? ???? ????? ?? ???? ??? ??? ????? ?? ?? ???? ?????? Alpha-, Beta-, Gammaproteobacteria ??? ??? ??? ???? ? 4~6? ??.
Alphaproteobacteria : ? ????? Alphaproteobacteria ?? ?? ?? ?? ??? ?????. ???? C?????? 9?? ??? ?????. ??? ???? ?? ???? T?????? 14?? ??? ????. ?? T ?????? Rhizobiales, Sphigomonadales , ??? Caulobacterales ? ??? ????? ??? ?????. T-1(97%), 5(96%), 15(96%), 78(98%)? 4?? ??? Rhizobiales ? ??? ??? ?????. Rhizobiaceae ?? ??? ??? ? ????? ?? ?????, ?????. ?? poly-Beta-hydroxybutyl ??? ?? ??? ????, ??? ????? ??? ???? ???? ??. ????( Rhizobium )? ?? ??? ?????? ????? ?? ??? ???? ?????? ????(???, 2004).
T-71(96%), 92(92%), 97(96%), 103(94%), 112(97%)? 5? ??? Sphingomonadales ? ??? ??? ?????(? 4). T-102(94%)? 1?? ??? Caulobacterales ? ??? ??? Phenylobacterium immobile ? ?????. ? ??? ?? ?? ??????, herbicide chloridazon ? ???? ???? ??? ??(Lingens, 1985). ??? ??? ???? ?? ??? ??????? ??? ?????? ??? ????? ??? ???? ???? ??? ?????.
Gammaproteobacteria : Gammaproteobacteria ? ??? Proteobacteria ??? ? ??? ?? ??? ?????. C ???? 5?? ???? ?????. ? ??? Pseudomonadaceae ? Pseudomonas ?? ??? 97%? ???? ?????, ? ??? ??? ???? ???? ?? ??? ??, ??? ??? ?????? ?????? ???? ?????. ? ??? ????, ??? Xanthomonadaceae ?? Rhodanobacter ?? 95% ???? ?? ??? ?????. T ???? 4?? ??? ?????. T ???? ???? ?? Pseudomonas ??? ?????. ??? Psuedomonadales , Pseudomonadaceae ?? ?? ??? ???. Pseudomonas ?? ??? ??? ?? ???? ??? ? ?? ?????, ?????, ??? ???? ???? ????? ??. ?? ??? ?? ??? ?? ??? ??? ? ??. ??? ????? ???? ????? ???? ??? ?? ????(???, 2004).
Betaproteobacteria : ? ??? ??? ???? C ?????? 2?, T ?????? 5?? ??? ?????. C ???? ????? ??? ??????, T ?????? ?? Nitrosomonadales ? Nitrosospira ??? ?????. T ????? ??? ???? Nitrosospira ??? 98%? ?? ???? ????. ? ??? ????? ?? ????, ???? ?????, ??? ?? ??? ??? ??. ?? ?? ????? ???? ?????? ???, ???? ??? ??? ?? ? ????(???, 2004). Comamonadaceae ? Acidovorax ?? ??? ??? 99%? ?? ???? ????. Oxalobacteraceae ? Janthinobacteria ? Nitrosospira sp. L115? 97%? ???? ?? ??? ?????, T ?????? C ??? ?? ?? ?? ??? ???? ???? ?? ????.
3. ???? ?? ??? ?? ???? ??
????? ???? ?? ??? ??? ?? ?? ????(metabolism-independent)? ?? ???(metabolism-dependent)? ? ??? ?? ? ? ??. ?? ????? ???? ?? ?? ?? ??? ???, ??? ?????, ????(biopigment) ?? ?? ???? ?????? ????? ??? ????? ?? ???. ??? ??? ???? ?? ???? ??? ???? ? ? ??? ?? ?? ? ???? ???? ??? ??? ? ??. ???? ??? ????? ?? ???? ?? ? ??? ??? ???? ???? ???? ??? ? ??. ??? ??? ?? ??? metal sulfide, zinc dust? ferric hydroxide ?? ?? ?????? ??? ??? ? ??(Gadd, 1992). ?? ????? ?? ????? ???? ?-???? ? ?? ??(siderophores)? ????? ??? Ga, Ni, Th, U ? Cu ?? ?? ?? ??? ?? ? ????. ?? ??? ??? Ag+? AG 0 ? ?????? ? ?? ?? ??? ? ?? ???? ?????. ??? ???? ?? ??? ???? ? ???? ?????? ??? ???? ??? ??, ??, ??? ?? ????(oxidation, reduction, methylation, demethylation) ???. Mecuric redutase? Hg 2+ ? ??? Hg 0 ? ?????. ???? ?? ???? ??????? ?? ?? ?? ???? ?? ??? ?? ??? ? ??(Bitton, 1992).
?? ???? ????? ????? ????? ?????? ????? ???? ? ?? ?? ???? ??? ? ?? ???? ?????(electron transport system), ??, ????? ?? ?? ???? ????? ???? ???? ?? ???? ???? ???? ???(??? ?, 1989), ?? ??, ???(metabolic poisons) ?? ???? ??? ?? ??? ???? ??? ???? ?? ??? ???. ???? ???? ?? ??? ???? ??? ??? ???? ??? ? ??? ??? ?? ?? ??? ?? ????? ??? ??? ?? ??? ????? ?? ??? ??? ??. Metallothionein? phytochelatin? ?? ???? ???? ??? Cu, Zn, Pb, Cd? ?? ???? ?? ??? Au? Ag ?? ?? ??? ???? ? ??? ? ??(???? ???, 2000).
? ??? ?????? ??? ????? ?????? ??? ???? ??? ?????? ?? ? ?????? ???? ??? ???? ?? ?? ?? ??? ???, ??? ?????, ????(biopigment) ?? ??????? ?? ???? ??? ??? ???? ???? ?? ? ??? ????.
??
???(Cd, Pb, Cu, Zn)? ??? ??? ???? ?? ???? ????? ?? ???? ??????? ? 22.6~75.7% ?? ?? ??? ???? ???? ??? ???? ??? ???? ??? ????.
??, ?? ??? ?? ?? ? ???? ?????? ?? ???? ?? ????? ???? ???? ???? ????? ???? ??? ????. ??, ???? ?? ????? ??? ????? ??? ??? ?? ??? ???? ????? ???? ???? ???? ??? ?? ??? ??? ?? ??????. ????? ??? ??? ???? ? ?? ? ???? ??? ? ?? ????? ??????, ? ???? ???? ???? ???? ??? ? ??? ?????.
?? ???? ??? ??? ???? ???? ?? ??? ???? ? ??? ???? ??? ?? ? ???? ????? ??? ??? ?? ????. ???? ??? ??? ??? ??? ? ??? ????? ???? ? ??, ?????? ?? ??? ??? ????.
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高效檢索全球?qū)＠?/div>專利匯是專利免費檢索,專利查詢,專利分析-國家發(fā)明專利查詢檢索分析平臺，是提供專利分析，專利查詢，專利檢索等數(shù)據(jù)服務(wù)功能的知識產(chǎn)權(quán)數(shù)據(jù)服務(wù)商。
我們的產(chǎn)品包含105個國家的1.26億組數(shù)據(jù)，免費查、免費專利分析。
分析報告專利匯分析報告產(chǎn)品可以對行業(yè)情報數(shù)據(jù)進行梳理分析，涉及維度包括行業(yè)專利基本狀況分析、地域分析、技術(shù)分析、發(fā)明人分析、申請人分析、專利權(quán)人分析、失效分析、核心專利分析、法律分析、研發(fā)重點分析、企業(yè)專利處境分析、技術(shù)處境分析、專利壽命分析、企業(yè)定位分析、引證分析等超過60個分析角度，系統(tǒng)通過AI智能系統(tǒng)對圖表進行解讀，只需1分鐘，一鍵生成行業(yè)專利分析報告。
